#NOPUBLISH 3/1/2024# Green synthesis, karakterisasi dan uji toksisitas senyawa basa schiff dari o-vanilin dan 2- aminobenzotiazol

ABSTRAK:

Schiff bases are compounds formed when primary amines react with aldehydes or ketones under specific conditions. Schiff base compounds have a characteristic C=N (imine) group. This study aims to determine the results of the synthesis of the Schiff base compound using the grinding method and the toxicity value of the Schiff base compound synthesized from o-vanillin and 2- aminobenzothiazole.
The method used in synthesizing Schiff base compounds from o-vanillin and 2-aminobenzothiazole compounds is using the grinding method with a time of 7 hours. Schiff base compound products were characterized using FTIR (Fourier Transform Infra Red) spectrophotometer and GC-MS (Gas Chromatography-Mass Spectrometry. The toxicity test of Schiff's base compound was carried out using the BSLT (Brine Shrimp Lethality Test) method. The LC50 value was used as a parameter for the toxic level of the Schiff base compound synthesized from o-vanillin and 2-aminobenzothiazole.
The resulting product is a greenish yellow solid, has a melting point between 165.6-166.6℃, insoluble in water and completely soluble in 2M NaOH solution. The FTIR spectra results show a typical absorption of the imine group (-HC=N-) with a wave number of 1595 cm-1. The GC-MS results showed a peak at a retention time of 44.539 minutes with a purity of 98.54% and a molecular ion m/z of 284, corresponding to the molecular weight of the Schiff base compound 6-methoxy-2-((benzothiazol-2-ilimino)methyl)phenol. Toxicity testing using the BSLT method on the Schiff base compound 6-methoxy-2-((benzothiazol-2-ilimino)methyl)phenol showed an LC50 value of 13.7021 ppm.

ABSTRACT:

Schiff bases are compounds formed when primary amines react with aldehydes orketones under specific conditions. Schiff base compounds have a characteristic C=N(imine) group. This study aims to determine the results of the synthesis of the Schiff basecompound using the grinding method and the toxicity value of the Schiff base compoundsynthesized from o-vanillin and 2- aminobenzothiazole.The method used in synthesizing Schiff base compounds from o-vanillin and 2-aminobenzothiazole compounds is using the grinding method witha time of 7 hours. Schiffbase compound products were characterized using FTIR (Fourier Transform Infra Red)spectrophotometer and GC-MS (Gas Chromatography-Mass Spectrometry. The toxicitytest of Schiff's base compound was carried out using the BSLT (Brine Shrimp LethalityTest) method. The LC50 value was used as a parameter for the toxic level of the Schiffbase compound synthesized from o-vanillin and 2-aminobenzothiazole.The resulting product is a greenish yellow solid, has a melting point between 165.6-166.6℃, insoluble in water and completely soluble in 2M NaOH solution. The FTIRspectra results show a typical absorption of the imine group (-HC=N-) with a wave numberof 1595 cm-1. The GC-MS results showed a peak at a retention time of 44.539 minuteswith a purity of 98.54% and a molecular ion m/z of 284, corresponding to the molecularweight of the Schiff base compound 6-methoxy-2-((benzothiazol-2-ilimino)methyl)phenol. Toxicity testing using the BSLT method on the Schiff basecompound 6-methoxy-2-((benzothiazol-2-ilimino)methyl)phenol showed an LC50 value of13.7021 ppm.

مستخلص البحث:

قوَاعِد َ شِيف ْ هِي َ مَر ِْ ِفِ ظِل لْكِيتُو َنَت ََكَّب ُ قَاعِدَة ِ شِيف ْ عَلَيَْتَوِي مُر ظُرُوف ٍ حمَُدَّدَةٍ. َِمَْمُوعَة ىC=Nَِِلَ َتَْدِيد ِ نتَائِج (إِميِنيْ) ُمَُيزَة ٌ . يهْدِف َ هَذَا اَلْبَحْث ِ إ ِْكَبَات ِ قَاعِدَة ِ شْ ِبِسْتِخْدَام ِ طَرِيقَة ِ اَلطَّحْن ِ وَقِيمَة ِ اَلسُّمِيَّة ِ لِمَر ْكَبَات ِ قَاعِدَة ِ شِيفَتَْلِيق ِ مَر ْْكَبَة ِ مِنيف ْ اَلْمَر-oفَانِيلِنيْ،٢-أَمِينُوبنزُوثِيَازُولْ.ْْكَبَات ِ قَاعِدَة ِ شِيف ْ مِنُ ِفِ توْلِيف ِ مَر اَلطَّرِيقَة ُ اَلْمُسْتَخْدَمَة–oفَانِيلِني ْ و٢- َأَمِينُوبنزُوثِيَازُول ْ تَسْتَخْدِم َ طَرِيقَة ِاَلطَّحْن ِ مَع َ وَقْت٠٩ِْ ِبِسْتِخْدَام ِ مَقَايِيس ِ اَلطَّيْف ِ اَلضَّو ْكَبَات ِ ا َْلَْسَاسِيَّة ِ لِشِيفَتََّ َتَْيِيز ُ مُنتَجَات ِ اَلْمَر دَقِيقَة ٍ . ِئِيFTIRِ َتَْت َ ا َْلَْمْرَاء)، و ْ ِبِْلَْشِعَّة ( َتَْوِيل ُ فُورِييِهGC-MSُ(كَرُومَاتُوغَرَافِيا اَلْغَاز- ِمِطْيَاف ُ اَلْكُتلَة َِتََّ إِجْرَاء َ اِخْتِبَار .) ٍَكَّب ِ قَاعِدَة ِ شِيف ْ ِبِسْتِخْدَام ِ طَرِيقَةاَلسُّمِيَّة ِ لِمُرBSLTٍ( اِخْتِبَار ٌ فتَك َ ا َْلَْمْربَِي ُّ اَلْمِلْحِيُّ) . يَتِم َّ اِسْتِخْدَام ُ قِيمَة50LCَِكَّب ِ قَاعِدَةكَمُعَلِمَة ٍ لِلْمُسْتوَى اَلسَّام ِ لِمُر ْْكَب ِ مِنشِيف ْ اَلْمَر- oفَانِيلِني ْ و٢-أَمِينُوبنزُوثِيَازُولْ.ََْ ُمُْضَرَّةٍ، و ََلََا نقْطَة ُ اِنْصِهَار ِ بَني اَلْمُنتَج ُ اَلنَّاتِج ُ عِبَارَة ً عَن ْ مَادَّة ٍ صُلْبَة ٍ صَفْرَاء٦٠٥٦١-٦٠٦٦١ٍدَرَجَة ٍ مِئَوِيَّة َِفِ ا ِ ، غَري َْ قَابلَة ٍ لِلذَّو َِبَن ِِفِ حمَْلُول ِ َتََامًا لْمَاء ِ وَقَابِلَة ً لِلذَّو َِبَنNaOH 2Mِنتَائِج َ أَطْيَافFTIRٌَلََا اِمْتِصَاصَنَُوذَجِي ٌّ لِمَجْمُوعَة ِ إِميِني)-HC=N-(بِرَقْم ِ مَوْجِي٥٩٥١سم-١.أَظْهَرَت ْ نتَائِجMS-GCًِ ِفِ وَقْت ِ اِحْتِفَاظ ذُرْوَة ِقَدْرِه٩٣٥٠٤٤ِدَقِيقَة ٍ بِنقَاوَة.٤٥٨٩٪ًوَأَيُو َنَت ُ جُزَيْئِيَّةm/zَتبلُغ٤٨٢ َِكَّب ِ قَاعِدَة، تتوَافَق َ مَع َ اَلْوَزْن ِ ا َْلُْزَيْئِي ِ لِمُر ْشِيف6-كْسِيمِيثُو-٢((ْبنزُوثِيَازُول-٢-َّإِلِيمِينُو ) مِيثِيل ْ ) اَلْفِينُول َ . أَظْهَر َ اِخْتِبَار ُ اَلسُّمِي ٍِ ِبِسْتِخْدَام ِ طَرِيقَة ةBSLT َْكَّب ِ قَاعِدَة ِ شِيفعَلَى مُر٦-كْسِيمِيثُو-٢-ْ(بنزُوثِيَازُول-٢- ًإِلِيمِينُو)مِيثِيلْ) اَلْفِينُول َ قِيمَة50LCُتبلُغ.١٢٠٧٣١ppm