Nashruddin, Miftahul Huda (2021) Analisis fungsional senyawa aktif eugenol daun lampes secara in silico sebagai kandidat agen kompetitif inhibitor ox-LDL dalam berikatan dengan SR-A dan CD-36. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
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Abstract
ABSTRAK:
Pada kondisi hiperkolesterolemia terjadi penumpukan LDL di pembuluh darah,
ROS dan NADPH mengoksidasi LDL sehingga terbentuklah ox-LDL. SRA dan CD36 merupakan reseptor yang berperan penting dalam terbentuknya sel busa. Reseptor tersebut dapat mengikat ox-LDL yang selanjutnya akan difagositosis oleh makrofag. Penumpukan ox-LDL dapat memicu terbentuknya sel busa.Adapun penatalaksanaan hiperkolesterolemia berupa obat golongan statin, akan tetapi obat tersebut menimbulkan beberapa efek samping yakni; peningkatan tekanan darah, dan kerusakan hati. Pengobatan tradisional mempunyai efek samping lebih kecil dan mempunyai manfaat yang lebih luas diandingkan dengan obat-obatan modern. Tumbuhan lampes (Ocimum sanctum linn) merupakan salah satu tanaman yang mengandung senyawa eugenol. Ox-LDL dapat dicegah menggunakan senyawa eugenol yang memiliki potensi hipolipidemia. Membuktikan potensi senyawa aktif daun lampes eugenol dengan inhibisi aterosklerosis secara in silico. Desain penelitian ini adalah penelitian deskriptif eksploratif, Penelitian ini dilakukan dengan beberapa tahapan meliputi: preparasi ligan, preparasi protein reseptor, uji Human Intestinal Absorption (HIA), Uji Lippinski Rule of five (Ro5), Uji Prediction of Activity Spectra for Substances
(PASS), penambatan molekular (Molecular Docking) dan visualisasi hasil docking. Hasil uji docking senyawa eugenol dengan SRA dan CD36 tumbuhan lampes (Ocimum sanctum linn) yang diujikan memiliki nilai binding affinity
terbaik yaitu -5 kkal/mol dan -5,8 kkal/mol. Senyawa eugenol memiliki efek hipolipidemia sedang. Eugenol juga memiliki kemampuan terabsorbsi baik di usus dan mampu menembus membrane sel sehingga dapat direkomendasikan menjadi kandidat obat antiaterosklerosis.
ABSTRACT:
In hypercholesterolemic conditions, LDL builds up in the blood vessels, ROS andNADPH oxidize LDL to form ox-LDL. SRA and CD36 are receptors that play animportant role in the formation of foam cells. These receptors can bind ox-LDLwhich will then be phagocytosed by macrophages. The buildup of ox-LDL cantrigger the formation of foam cells. The management of hypercholesterolemia isin the form of statin drugs, but these drugs cause several side effects, namely;increased blood pressure, and liver damage. Traditional medicine has fewer sideeffects and has a wider range of benefits than modern medicine. Basil leaf(Ocimum sanctum linn) is a plant that contains eugenol compounds. Ox-LDL canbe prevented by using eugenol compounds which have the potential forhypolipidemia. Proving the potency of the active compound of basil eugenol leafwith in silico inhibition of atherosclerosis. The design of this research isdescriptive exploratory research. This research was carried out in several stagesincluding: ligand preparation, receptor protein preparation, Human IntestinalAbsorption (HIA) test, Lippinski Rule of five (Ro5) test, Prediction of ActivitySpectra for Substances (PASS) test, Molecular Docking and visualization ofdocking results. The results of the docking test for eugenol compounds with SRAand CD36 of the lampes plant (Ocimum Sanctum Linn) tested had the best bindingaffinity values, namely -5 kcal/mol and -5.8 kcal/mol. Eugenol compounds have ahypolipidemic effect. Eugenol also has the ability to be well absorbed in theintestine and is able to penetrate cell membranes so that it can be recommended asan antiatherosclerosis drug candidate.
Item Type: | Thesis (Undergraduate) |
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Supervisor: | Rachmawati, Ermin and Rahmah, Zainabur and Rahmawati, Ana |
Keywords: | Eugenol; lampes (Ocimum sanctum linn); SRA & CD36; in silico; antiaterosklerosis Eugenol; Basil leaf (Ocimum sanctum linn); SRA & CD36; in silico; antiatherosclerosis |
Subjects: | 11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiorespiratory Medicine and Haematology > 110201 Cardiology (incl. Cardiovascular Diseases) |
Departement: | Fakultas Kesehatan dan Ilmu Kedokteran > Jurusan Pendidikan Dokter |
Depositing User: | Miftahul Huda Nashruddin |
Date Deposited: | 19 Sep 2023 14:12 |
Last Modified: | 19 Sep 2023 14:12 |
URI: | http://etheses.uin-malang.ac.id/id/eprint/56347 |
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