Mayangsari, Mayangsari (2016) Pemurnian enzim Amilase kasar dari bakteri Amilolitik endogenous bekatul secara parsial menggunakan ammonium sulfat. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
![]() |
Text (Fulltext)
10630076.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (4MB) |
Abstract
INDONESIA:
Bakteri amilolitik adalah bakteri yang memproduksi enzim amilase yang mampu memecah pati menjadi glukosa. Bekatul yang kaya akan amilosa, kemungkinan bahwa bakteri amilolitik mampu hidup dalam lingkungan yang kaya akan amilosa. Pengendapan merupakan proses awal dalam pemurnian enzim, bertujuan untuk memisahkan protein enzim dari protein-protein lainnya. Penelitian ini bertujuan untuk memurnikan enzim amilase kasar dari bakteri amilolitik endogenous bekatul secara parsial menggunakan amonium sulfat. Penelitian ini diawali dengan pemurnian ekstrak kasar enzim amilase secara fraksinasi (20–40% (F1), 40–60% (F2), dan 60–80% (F3)) menggunakan ammonium sulfat dan dialisis dengan kantong selofan. Hasil fraksinasi dengan amonium sulfat aktivitas tertinggi kemudian ditentukan aktivitas spesifiknya. Hasil penelitian menunjukkan hasil pemurnian dengan fraksinasi amonium sulfat menghasilkan (Paralelisme) aktivitas berturut-turut 6,24 × 10-2 U/mL,7,03 × 10-2 U/mL, dan 5,97 × 10-2 U/mL. Aktivitas spesifik amilase dari fraksinasi tertinggi (F2) sebesar 3,71 x 10-5 U/mL.
ENGLISH:
Amylolytic bacteria are bacteria that produce amylase enzymes capable of breaking down starch into glucose. Bran which is rich in amylose content, amylolytic bacteria are able to live in an environment which is rich in amylose. Precipitation is the first process in the purification of the enzyme, aiming to separate the protein of the enzyme from other proteins. This study aimed to purify the amylase enzyme from bacteria coarse bran partially endogenous amylolytic using ammonium sulfate. This study begins with the purification of crude extract of enzyme amylase in fractionation (20–40 % (F1), 40–60 % (F2), and 60–80 % (F3)) using ammonium sulfate and dialysis with cellophane bag. The results fractionation with ammonium sulfate, the highest activity is then determined specific activity. The results showed that the purification with ammonium sulfate fractionation produced successive activities of 6.24 × 10-2 U/mL, 7.03 × 10-2 U/mL, and 5.97 × 10-2 U/mL. The amylase specific activity of the highest fractionation was (F2) of 3.71 x 10-5 U/mL.
Item Type: | Thesis (Undergraduate) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Supervisor: | Jannah, Akyunul and Fauziyah, Begum | |||||||||
Contributors: |
|
|||||||||
Keywords: | Enzim Amilase; Bakteri amilolitik; Pemurnian Enzim; Amylase Enzyme; Amylolitic Bacteria; Purification Enzyme | |||||||||
Departement: | Fakultas Sains dan Teknologi > Jurusan Kimia | |||||||||
Depositing User: | Desy Putri Andika | |||||||||
Date Deposited: | 21 Jul 2016 12:04 | |||||||||
Last Modified: | 21 Jul 2016 12:04 | |||||||||
URI: | http://etheses.uin-malang.ac.id/id/eprint/3416 |
Downloads
Downloads per month over past year
Actions (login required)
![]() |
View Item |