Uji daya hambat larutan formalin dan fenol terhadap jamur aspergillus sp. hasil isolasi dari cairan perendam kadaver, air keran dan udara di laboratorium anatomi FKIK UIN Maliki Malang

Abstrak :

Kadaver dan cairan pengawetnya sering ditemukan adanya pertumbuhan jamur. Tujuan dari penelitian ini ialah untuk mengetahui adanya pertumbuhan jamur Aspergillus sp. pada cairan perendam kadaver, air keran, udara di ruang penyimpanan cadaver dan ruang praktikum anatomi. Untuk mengetahui daya hambat fungi dari larutan formalin 5%, formalin 5% + fenol 5%, formalin 7,5%, dan formalin 7,5% + fenol 5%. Penelitian ini menggunakan desain penelitian eksperimental laboratorium sungguhan. Sampel jamur yang digunakan ialah jamur Aspergillus sp. yang berasal dari cairan perendam kadaver dan cairan perendam organ kadaver, air keran, udara yang ada di ruang penyimpanan kadaver dan ruang praktikum anatomi. Jamur yang teridentifikasi sebagai Aspergillus sp. dilakukan pembuatan biakan murni pada Potato Dextrose Agar (PDA). Kemudian dilakukan proses uji daya hambat menggunakan Metode Difusi Kertas Cakram. Hasil penelitian menunjukkan bahwa rerata zona hambat terbesar yang terbentuk ialah pada kombinasi cairan formalin 7,5% dan fenol 5% dan termasuk dalam zona hambat yang sangat kuat menurut interpretasi Davis dan Stout (1971). Hasil statistik menunjukkan hasil yang sangat bermakna dengan uji ANOVA satu arah (p=0,000) dan hasil uji Post Hoc Bonferonni menunjukkan terdapat perbedaan yang bermakna antara kelompok kombinasi formalin 7,5% dan fenol 5% dengan kelompok perlakuan lain. Dapat disimpulkan Ditemukan jamur Aspergillus s. pada hasil isolasi cairan perendam kadaver, udara di ruang penyimpanan kadaver dan ruang Praktikum Anatomi FKIK UIN Malang. Konsentrasi larutan paling kuat dalam menghambat pertumbuhan jamur ialah kombinasi larutan formalin 7,5% dan fenol 5% .

Abstract :

Cadaver and preservative fluid are often found in the presence of fungal growth. The purpose of this study is to determine the growth of aspergillus sp fungus. in cadaver preservative fluids, tap water, air of cadaver storage room and anatomical practicum room. To know the inhibition zone of formaldehyde solution 5%, formalin 5% and phenol 5%, formalin 7.5%, , and combination of formalin 7.5% + phenol 5%. The sample used is Aspergillus sp fungal derived from cadaver preservatif fluid and cadaver organ fixation fluid, tap water, air in cadaver storage room and anatomical practicum room. The fungal identified as Aspergillus sp. was inoculated on Potato Dextrose Agar. Then tested with various presservative solutions to investigate the inhibitory effect of Asperillus sp. growth using the Disc Paper Diffusion Method . The results showed that the largest average inhibition zone formed was in a combination of formalin 7.5% and phenol 5% and included in a very strong inhibition zone according to the interpretation of Davis and Stout (1971). The statistics showed very meaningful results with a one-way ANOVA test (p=0.000) and Post Hoc Bonferonni test results showed significant differences between the 7.5% formaldehyde and 5% phenol combination groups with other treatment groups. This research concluded that Aspergillus sp. was found in the isolation of cadaver preservative fluids, air of cadaver storage room and Anatomical Practicum room of FKIK UIN Malang. The strongest concentration of solution in inhibit the growth of fungi is a combination of formalin 7.5% and phenol 5%