Nafiandary, Mely (2019) Pengaruh ekstrak buah Tin (Ficus carica l.) sebagai antioksidan terhadap ketebalan epitel dan diameter lumen tubulus proksimal ginjal mencit betina yang dipapar rhodamin B. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
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Abstract
INDONESIA:
Senyawa radikal bebas dalam rhodamin B tidak dapat termetabolisme di hati, sehingga mempengaruhi proses reabsorbsi di ginjal yang menyebabkan penebalan epitel tubulus dan penyempitan diameter lumen tubulus. Buah tin mengandung polifenol sebagai senyawa antioksidan yang mampu menghambat radikal bebas dengan cara menyumbangkan satu elektron kepada elektron yang tidak berpasangan dalam radikal bebas. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak buah tin (Ficus carica L.) sebagai antioksidan terhadap ketebalan epitel dan diameter lumen tubulus mencit betina yang dipapar rhodamin B, serta mengetahui Effective Dose 50 (ED50) dari ekstrak buah tin dalam mempengaruhi ketebalan epitel dan diameter lumen tubulus. Penelitian ini dilakukan dengan menggunakan 25 ekor mencit betina yang dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif diberikan rhodamin B 0,08 mg/25gBB, kontrol positif diberikan rhodamin B 0,08 mg/25gBB dan vitamin C 1,3 mg/25g BB, kelompok perlakuan I (P1) diberikan rhodamin B 0,08 mg/25gBB dan ekstrak buah tin 1,75 mg/25gBB, kelompok perlakuan II (P2) diberikan rhodamin B 0,08 mg/25gBB dan ekstrak buah tin 3,5 mg/25gBB, kelompok perlakuan III (P3) diberikan rhodamin B 0,08 mg/25gBB dan ekstrak buah tin 7 mg/25gBB. Analisis data dilakukan dengan uji Kruskal-wallis dengan uji Mann-whitney sebagai uji lanjutan. Hasil penelitian menunjukan pemberian ekstrak buah tin berpengaruh secara signifikan terhadap ketebalan epitel tubulus (nilai p=0,000) dan diameter lumen tubulus (nilai p=0,000) dengan menunjukan pengecilan ketebalan epitel serta pembesaran diameter lumen tubulus ginjal mencit. Pengujian ED50 dari ekstrak buah tin didapatkan hasil 2,39 mg/25gBB.
ENGLISH:
Free radical compounds in rhodamine B cannot be metabolized in the liver, so they enter the excretion system and affect the reabsorption process in the kidney which causes the thickening of the tubule epithelium and the narrowing of the tubule lumen diameter. Figs contain polyphenols as antioxidant compounds that can inhibit free radicals by donating one electron to unpaired electrons in free radicals, so that the number of free radicals decreases. The purpose of this study was to determine the effect of figs (Ficus carica L.) extract as an antioxidant on the thickness of the epithelium and lumen diameter of female mice exposed rhodamine B, and to determine the effective dose 50 (ED50) of figs extract in influencing the thickness of epithelium and lumen diameter tubule. This research was conducted using 25 female mice which were divided into 5 groups, namely the negative control group which was given rhodamine B 0.08 mg/25gBW, the positive control group which was given rhodamine B 0.08 mg/25gBW and vitamin C 1.3 mg/25gBW, the treatment group I (P1) which was given rhodamine B 0.08 mg/25gBW and figs extract 1.75 mg/25gBW, the treatment group II (P2) which were given rhodamine B 0.08 mg/25gBW and figs extract 3.5 mg/25gBW, and the treatment group III (P3) which was given rhodamine B 0.08 mg/25gBW and figs extract 7 mg/25gBW. Data analysis was performed using the Kruskal-wallis test with the Mann-whitney test as a follow-up test. The results showed that the distribution of figs extracts had a significant effect on the thickness of tubule epithelium (p=0,000) and the lumen diameter of tubule (p=0,000) by showing a reduction in epithelial thickness and enlargement of lumen diameter of the kidney tubule. ED50 test of figs extracts obtained 2.39 mg/25gBW.
Item Type: | Thesis (Undergraduate) | |||||||||
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Supervisor: | Muti’ah, Roihatul and Dianti, Meilina Ratna | |||||||||
Contributors: |
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Keywords: | Ficus carica L.; rhodamin B; ketebalan epitel; diameter lumen; tubulus ginjal epithelial thickness; lumen diameter; kidney tubule | |||||||||
Departement: | Fakultas Sains dan Teknologi > Jurusan Farmasi | |||||||||
Depositing User: | Marifa Nisa | |||||||||
Date Deposited: | 11 Apr 2020 13:48 | |||||||||
Last Modified: | 11 Apr 2020 13:48 | |||||||||
URI: | http://etheses.uin-malang.ac.id/id/eprint/16416 |
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