Pengaruh suhu dan pH terhadap aktivitas enzim selulase dari kultur campuran Trichoderma sp., Gliocladium sp. dan Botrytis sp. yang ditumbuhkan pada media kulit pisang

Fitrianti, Rodiyatul (2014) Pengaruh suhu dan pH terhadap aktivitas enzim selulase dari kultur campuran Trichoderma sp., Gliocladium sp. dan Botrytis sp. yang ditumbuhkan pada media kulit pisang. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.

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Abstract

INDONESIA:

Industri enzim telah berkembang pesat dan menempati posisi penting dalam bidang industri , khususnya enzim selulase. Selulase merupakan enzim yang potensial digunakan dalam hidrolisis bahan berselulosa menjadi gula-gula sederhana. Aplikasi enzim selulase sangat luas dalam bidang industri. Campuran enzim dari beberapa kapang mampu memperbaiki komposisi enzim selulase menjadi lebih seimbang untuk menghidrolisis selulosa. Campuran kapang Trichoderma sp., Gliocladium sp., dan Botrytis sp. mampu menghasilkan enzim selulase dengan zona bening sebesar 9,13 cm. Kulit pisang dapat dimanfaatkan sebagai media produksi enzim selulase dari campuran kapang Trichoderma sp., Gliocladium sp. dan Botrytis sp. Untuk memaksimalkan aktivitas enzim selulase dari campuran Trichoderma sp, Gliocladium sp dan Botrytis sp. perlu dilakukan optimalisasi aktivitas enzim selulase dengan mengkombinasikan beberapa variabel yang dapat mempengaruhi aktivitas enzim diantaranya suhu, pH, konsentrasi enzim dan substrat, aktivator dan inhibitor. Penelitian ini bertujuan untuk mengetahui pengaruh suhu, pH dan interaksi suhu dan pH terhadap aktivitas enzim selulase dari campuran kapang Trichoderma sp, Gliocladium sp. dan Botrytis sp. yang ditumbuhkan pada media kulit pisang.

Penelitian ini adalah penelitian eksperimental dengan menggunakan rancangan acak lengkap (RAL) pola faktorial dengan dua faktor. Faktor pertama adalah variasi suhu yang terdiri dari 3 taraf yaitu 40°C, 50°C, dan 60°C. Faktor kedua adalah variasi pH yang terdiri dari tiga taraf yaitu 4, 5, dan 6. Masing-masing faktor dilakukan pengulangan sebanyak 3 kali. Data yang diperoleh dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Apabila perlakuan berpengaruh nyata terhadap parameter maka dilanjutkan dengan Uji Duncan Multiple Test (DMRT).

Hasil penelitian menunjukkan bahwa (1) Suhu berpengaruh terhadap aktivitas enzim selulase dari campuran kapang Trichoderma sp., Gliocladium sp., dan Botrytis sp. Aktivitas enzim selulase tertinggi diperoleh dari perlakuan suhu 50°C dengan aktivitas enzim selulase berturut-turut sebesar 27,25 U/ml. (2) pH berpengaruh terhadap aktivitas enzim selulase dari kultur campuran kapang Trichoderma sp., Gliocladium sp., dan Botrytis sp. Aktivitas enzim selulase tertinggi diperoleh dari perlakuan pH 6 dengan aktivitas enzim selulase sebesar 24,51 U/ml.(3) Interaksi suhu dan pH berpengaruh terhadap aktivitas enzim selulase dari campuran kapang Trichoderma sp., Gliocladium sp., dan Botrytis sp. Aktivitas enzim selulase tertinggi diperoleh dari perlakuan suhu 50°C pada pH 6 dengan nilai aktivitas enzim selulase sebesar 32,56 U/ml.

ENGLISH:

The Enzyme industry has been growing rapidly and occupies an important position in the field of industry, particularly cellulases. Cellulase is an enzyme potentially used in the cellulated hydrolysis materials into simple sugars. Cellulase enzymes are broadly utilized in industry A mixture of enzymes from several fungi is able to repair the composition cellulase enzymes becoming more balanced to hydrolyze cellulose. Mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. fungus can produce the cellulase enzyme with a clear zone of 9.13 cm. Banana peels can be used as a medium for the production of cellulase enzyme from a mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. fungus. To maximize the activity of the cellulase enzyme mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. need to optimize the activity of cellulase enzymes by combining several variables that can affect enzyme activity including temperature, pH, concentration of enzyme and substrate, activator and inhibitor. The purpose of this research was to examine the effect of temperature, pH and temperature interactions and pH on the activity of cellulase enzyme mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. grown on banana peel medium.

This research was an experimental study using a completely randomized factorial design with two factors. The first factor was the temperature variation consisting of 3 levels including 40°C, 50°C, and 60°C. The second factor was the pH variation consisting of three levels including 4, 5, and 6. The researcher performed 3 times repetition for each factor. The data were analyzed by Analysis Of Variance (ANOVA). If the treatment significantly affected the parameters than it would be followed by Duncan's Multiple Test (DMRT).

The results showed that (1) The temperature affected the activity of cellulase enzyme from a mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. fungus. The highest cellulase enzyme activity was obtained from the treatment of 50°C with cellulase enzyme activity of 27,25 U/ml. (2) pH affected the activity of the cellulase enzyme from the mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. fungus. The highest cellulase enzyme activity obtained from pH 6 treatment with cellulase enzyme activity of 24,51 U/ml. (3) The interaction of temperature and pH affect the activity of the cellulase enzyme from the mixture of Trichoderma sp., Gliocladium sp. and Botrytis sp. fungus. The highest activity of cellulase enzymes derived from 50°C temperature treatment at pH
6 with cellulase enzyme activity of 32.56 U/ml.

Item Type: Thesis (Undergraduate)
Supervisor: Harianie, Liliek and Ahmad, Mujahidin
Keywords: Suhu; pH, Enzim Selulase; Trichoderma sp; Gliocladium sp; Botrytis sp; Temperature; pH; enzymes cellulase; Trichoderma sp; Gliocladium sp; Botrytis sp
Subjects: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
Departement: Fakultas Sains dan Teknologi > Jurusan Biologi
Depositing User: Chusnul Faida Ulfa
Date Deposited: 22 Jul 2015 01:48
Last Modified: 22 Jul 2015 01:48
URI: http://etheses.uin-malang.ac.id/id/eprint/453

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