Kumalasari, Diah (2014) Uji aktivitas antibakteri asam lemak hasil hidrolisis minyak Mikroalga Chlorella sp. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
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Abstract
INDONESIA:
Chlorella sp. merupakan mikroalga uniselular berukuran mikroskopis yang banyak mengandung minyak organik yang dapat dihidrolisis menjadi asam lemak. Asam lemak merupakan salah satu komponen aktif dalam mikroalga yang diduga berperan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri asam lemak hasil hidrolisis minyak mikroalga Chlorella sp. terhadap bakteri Escherichia coli dan Staphylococcus aureus. Isolasi minyak Chlorella sp. dilakukan dengan metode Soxhletasi dengan pelarut n-heksana. Minyak Chlorella sp. dihidrolisis dengan KOH 12% dalam pelarut metanol untuk mendapatkan asam lemak. Asam lemak yang dihasilkan diuji aktivitas antibakteri terhadap bakteri E. coli dan S. aureus menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa rendemen Soxhletasi minyak Chlorella sp. adalah sebesar 6,28%. Hidrolisis minyak Chlorella sp. menghasilkan rendemen sebesar 69,57%. Asam lemak memiliki aktivitas antibakteri terhadap bakteri S. aureus, tetapi tidak terhadap bakteri E. coli. Zona hambat asam lemak Chlorella sp. terhadap bakteri S. aureus pada konsentrasi 0,5; 1; 1,5; 2; dan 2,5% secara berturut-turut adalah 1,8; 1,9; 3,2; 3,5; dan 3 mm.
ENGLISH:
The Chlorella sp. is a microscopic unicellular microalgae that contains organic oils which can be hydrolyzed into fatty acids. Fatty acids is one of the active components in microalgae which allegedly acted as an antibacterial. The aim of this study is to determine the antibacterial activity of fatty acids from the hydrolysis process of microalgae Chlorella sp. oil against Escherichia coli and Staphylococcus aureus. Insulating oil Chlorella sp. performed by the Soxhlet method with n-hexane. Chlorella sp. oil was hydrolyzed with 12 % KOH in methanol to obtain fatty acids. The antibacterial activity of these fatty acids was tested against E. coli and S. aureus using the disc diffusion method. The result showed that the yield of oil Soxhlet Chlorella sp. was equal to 6,28 %. Hydrolysis of Chlorella sp. oil produced a yield of 69,57 %. Fatty acids have antibacterial activity against S. aureus bacteria, but not against the bacteria E. coli. Fatty acids inhibition zone from Chlorella sp. against the bacteria S. aureus at the concentration 0,5; 1; 1,5; 2; and 2,5% respectively are 1,8; 1,9; 3,2; 3,5; and 3 mm.
Item Type: | Thesis (Undergraduate) | |||||||||
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Supervisor: | Fasya, A. Ghanaim and Adi, Tri Kustono | |||||||||
Contributors: |
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Keywords: | Mikroalga Chlorella sp.; asam lemak; ekstraksi Soxhlet; aktivitas antibakteri; Microalgae Chlorella sp.; Fatty acids; Soxhlet extraction; antibacterial activity | |||||||||
Departement: | Fakultas Sains dan Teknologi > Jurusan Kimia | |||||||||
Depositing User: | Heni Kurnia Ningsih | |||||||||
Date Deposited: | 12 Jan 2018 14:54 | |||||||||
Last Modified: | 12 Jan 2018 14:54 | |||||||||
URI: | http://etheses.uin-malang.ac.id/id/eprint/8445 |
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