Pengaruh pemberian ekstrak etanol 70% daun dandang gendis (Clinacanthus nutans) terhadap kadar CK-MB (creatine kinase-myocardial band) jantung tikus model diabetes melitus

INDONESIA:

Hiperglikemia akibat Diabetes Melitus memicu terjadinya stress oksidatif di berbagai organ termasuk jantung dimana terjadi peningkatan enzim CK-MB (Creatine Kinase-Myocardial Band). Tanaman Clinacanthus nutans yang banyak dijumpai di Indonesia memiliki potensi antioksidan dan antidiabetik. Penelitian ini memiliki tujuan untuk mengetahui pengaruh pemberian ekstrak etanol 70% daun Clinacanthus nutans terhadap kadar CK-MB jantung tikus model DM. Penelitian ini menggunakan 30 ekor tikus Wistar Rattus novergicus jantan. Induksi DM menggunakan Streptozotosin 45 mg/kgBB intraperitoneal. Tikus didiagnosis DM jika kadar GDP tujuh hari pasca induksi Streptozotosin >200 mg/dL. Tikus dibagi menjadi 5 kelompok, yakni K1 (tikus kontrol normal), K2 (tikus kontrol DM), P1 (tikus DM yang diberikan ekstrak C. nutans 100 mg/kgBB), P2 (tikus DM yang diberikan ekstrak C. nutans 200 mg/kgBB) dan P3 (tikus DM yang diberikan ekstrak C. nutans 400 mg/kgBB). Pada akhir penelitian, serum darah tikus diambil untuk pengukuran kadar CK-MB total dengan menggunakan metode ELISA. Hasil penelitian menunjukkan K2 memiliki kadar CK-MB jantung yang signifikan lebih tinggi dibandingkan K1, kelompok tikus P2 dan P3 memiliki kadar CK-MB jantung yang signifikan lebih rendah dibandingkan K2, sedangkan kelompok P1 mengalami penurunan kadar CK-MB tetapi tidak signifikan dibandingkan K2. Kesimpulan penelitian ini yakni pemberian ekstrak etanol 70% daun Clinacanthus nutans mampu menurunkan kadar CK-MB jantung tikus model DM.

ENGLISH:

Hyperglycemia caused by Diabetes Mellitus triggers oxidative stress in various organs, including the heart, which leads to an increase in CK-MB (Creatine Kinase-Myocardial Band) enzyme levels. Therefore, Clinacanthus nutans has antioxidant and antidiabetic potential. This research determines the effect of 70% ethanol extract of C. nutans leaves on CK-MB levels in heart of diabetic rats. Rats were induced diabetic models intraperitoneally with streptozotocin 45 mg/kgBW. Diabetic rats determined when the blood glucose level reach up to 200 mg/dL. This study used thirty male Wistar rats divided into 5 samples : normal control, diabetic control, and diabetic rats administered with C.nutans extract at 100 mg/kgBW, 200 mg/kgBw and 400 mg/kgBw. At the end of the study, rat blood serum was collected to measure total CK-MB levels using the ELISA method. The results showed that diabetic control rats had significantly higher cardiac CK-MB levels than normal rats. Groups P2 and P3 exhibited significantly lower CK-MB levels compared to diabetic control rats, whereas group P1 showed a decrease that was not statistically significant. The conclusion is administration of 70% ethanol extract of Clinacanthus nutans leaves was able to reduce cardiac CK-MB levels in diabetic rat models.