Pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L.) terhadap anatomi dan histologi hepar mencit betina (Mus musculus) yang diinduksi 7,12 dimetilbenz (α) antrasen (DMBA) secara in vivo

Bidaah, Asnal (2013) Pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L.) terhadap anatomi dan histologi hepar mencit betina (Mus musculus) yang diinduksi 7,12 dimetilbenz (α) antrasen (DMBA) secara in vivo. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.

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Abstract

INDONESIA:

Keadaan normal sel hepar akan membelah diri jika ada penggantian sel-sel hepar yang telah mati dan rusak. Sebaliknya sel kanker akan membelah terus sehingga terjadi penumpukan sel baru yang menimbulkan desakan dan merusak jaringan normal pada hepar. Kondisi di atas dapat dicegah dengan memanfaatkan bahan aktif dari tumbuhan, salah satunya adalah sirsak. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol daun sirsak (Annona muricata L.) terhadap anatomi dan histologi hepar mencit betina (Mus musculus) yang diinduksi 7,12 dimetilbenz (α) antrasen (DMBA) secara in vivo.

Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 ulangan. Perlakuan yang digunakan adalah kontrol negatif (diberi pelarut ekstrak dan pelarut DMBA), kontrol positif (diberi pelarut ekstrak dan DMBA), perlakuan yang diberi DMBA dan diberi ekstrak Daun Sirsak dengan 4 dosis yang berbeda yaitu 100mg/kgBB, 150mg/kgBB, 200mg/kgBB, 250mg/kgBB selama 6 minggu. Parameter yang diamati dalam penelitian ini adalah anatomi dan histologi hepar. Data yang diperoleh dianalisis menggunakan ANOVA. Apabila hasil ANOVA menunjukkan pengaruh, maka dilanjutkan dengan uji Duncan 1%.

Hasil penelitian menunjukkan bahwa pemberian ekstrak Daun Sirsak (Annona muricata L) berpengaruh terhadap anatomi dan histologi hepar mencit betina (Mus musculus) yang diinduksi DMBA. Anatomi hepar ditunjukkan dengan warna hepar yang pucat dan munculnya jaringan parut pada hepar. Sedangkan histologi sel hepar ditunjukkan dengan nilai rerata tingkat nekrosis tahap akhir (piknosis) hepar pada perlakuan K+= 31,1%, D1= 14,1%, D2= 3,76%, D3= 2,36% dan K-= 0,54%, dan nilai rerata diameter inti sel hepar pada perlakuan K+= 3,24µ m, D1= 4,44µ m, D2= 6,51µ m, D3= 7,03µ m, dan K-= 3,24µ m.

ENGLISH:

Normal circumstances the liver cells will divide if there is replacement of liver cells that have died and broken. Instead cancer cells will continue to divide resulting in the accumulation of new cells that cause pressure and damage the normal tissue of the liver. Acetogenin compounds in soursop leaves can inhibit ATP transport and the formation of NADH which is selective only to the cancer cells. The purpose of this study was to determine the effect of ethanol extract of leaves of soursop (Annona muricata L.) on hepatic histology and anatomy of female mice (Mus musculus) induced dimetilbenz 7.12 (α) antrasen (DMBA) in vivo.

This study was an experimental study using a completely randomized design (CRD) with 6 treatments and 4 replications. The treatment used is the negative control (given solvent and solvent extracts DMBA), positive control mice (given solvent extract and DMBA) and DMBA treatment given and given extracts of Soursop Leaf with 4 different doses ie 100mg/kgBB, 150mg/kgBB, 200mg / kg, 250mg/kgBB for 6 weeks. Parameters observed in this study is the anatomy and histology of the liver. Data were analyzed using ANOVA. If the results of the analysis of variance showed significant effect, then followed by Duncan's test 1%.

Results of this study showed that the leaf extract Soursop (Annona muricata L) give effect to the anatomy and histology of the liver of female mice (Mus musculus) DMBA-induced. Anataomi hepatic liver indicated by the pale color and appearance of scarring of the liver suggests that liver cancer experience. Histological liver cells indicated by the mean level of the final stages of necrosis (piksosis) on treatment liver K + = 31.1%, D1 = 14.1%, = 3.76% D2, D3 = 2.36% and K-= 0, 54%. While the average value of the diameter of the cell nucleus in the treatment of hepatic K + µ m = 3.24, D1 = 4.44 µ m, D2 = 6.51, D3 = 7.03 µ m, and K-= 3.24 µ m.

Item Type: Thesis (Undergraduate)
Supervisor: Susilowati, Retno and Abidin, Munirul
Keywords: Ekstrak; Daun Sirsak; Anatomi Hepar; Histologi Hepar; 7,12 Dimetilbenz (α) Antrasen (DMBA); Extract; Soursop Leaf; Liver Anatomy; Liver Histology
Subjects: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060807 Animal Structure and Function
Departement: Fakultas Sains dan Teknologi > Jurusan Biologi
Depositing User: Saputra Edi
Date Deposited: 30 Jul 2015 04:00
Last Modified: 30 Jul 2015 04:00
URI: http://etheses.uin-malang.ac.id/id/eprint/579

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