Lestari, Sri (2013) Pengaruh jenis eksplan dan konsentrasi IBA (Indole Butyric Acid) terhadap pertumbuhan dan kadar metabolisme sekunder (stigmasterol dan sitosterol)kalus purwoceng (Pimpinella alpine molk.) pada media MS. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
|
Text (Introductio)
08620043 Pendahuluan.pdf Download (649kB) | Preview |
|
|
Text (Abstract: Indonesia)
08620043 Indonesia.pdf Download (88kB) | Preview |
|
|
Text (Abstract: English)
08620043 Inggris.pdf Download (13kB) | Preview |
|
|
Text (Abstract: Arabic)
08620043 Arab.pdf Download (219kB) | Preview |
|
|
Text (Chapter 1)
08620043 Bab1.pdf Download (410kB) | Preview |
|
|
Text (Chapter 2)
08620043 Bab 2.pdf Download (730kB) | Preview |
|
|
Text (Chapter 3)
08620043 Bab 3.pdf Download (579kB) | Preview |
|
|
Text (Chapter 4)
08620043 Bab 4.pdf Download (982kB) | Preview |
|
|
Text (Chapter 5)
08620043 Bab 5.pdf Download (230kB) | Preview |
|
|
Text (References)
08620043 Daftar Pustaka.pdf Download (149kB) | Preview |
|
![]() |
Other (Appendices)
Lampiran.rar Download (1MB) |
Abstract
INDONESIA:
Purwoceng (Pimpinella alpine Molk.) merupakan tanaman obat Indonesia yang memiliki khasiat sebagai afrodisiak, diuretik dan tonik. Keberadaan tanaman ini telah langka dan dikategorikan hampir punah. Teknik kultur jaringan dapat digunakan sebagai alternatif pembiakan dan sarana produksi metabolit sekunder. Pada penelitian ini digunakan zat pengatur tumbuh IBA (Indole Butyric Acid) dalam media MS untuk induksi kalus dan meningkatkan kadar metabolit sekunder berupa stigmasterol dan sitosterol. Tujuan dari penelitian ini adalah mengetahui pengaruh jenis eksplan dan konsentrasi IBA terhadap pertumbuhan dan kadar metabolit sekunder (stigmasterol dan sitosterol) kalus Purwoceng (Pimpinella alpine molk.) pada media MS.
Penelitian ini termasuk dalam penelitian eksperimental dengan rancangan acak lengkap (RAL) faktorial, perlakuan berupa jenis eksplan dan konsentrasi IBA. Eksplan berupa daun dan petiol sedangkan konsentrasi IBA yang digunakan yaitu 0 mg/l, 3 mg/l, 5 mg/l dan 7 mg/l. Uji kadar stigmasterol dan sitosterol dilakukan dengan menggunakan kromatografi kolom. Data penelitian berupa data kualitatif (pengamatan secara visual meliputi hari muncul kalus dan morfologi kalus) dan kuantitatif (berat kalus, kadar sitosterol dan stigmasterol). Data kualitatif dianalisis secara deskriptif sedangkan data kuantitatif dianalisis dengan ANAVA (Analisis variansi) 2 jalur, apabila ada pengaruh maka dilanjutkan dengan DMRT 5%.
Hasil penelitian menunjukkan bahwa terdapat pengaruh yang nyata dari jenis eksplan dan konsentrasi IBA terhadap pertumbuhan dan kadar metabolit sekunder kalus Purwoceng. Eksplan daun dengan IBA 3 mg/l merupakan perlakuan terbaik yang menghasilkan pertumbuhan tercepat yaitu 10 hst telah muncul kalus dengan warna hijau kekuningan, memiliki tekstur kompak, serta persentase pertumbuhan kalus tertinggi daun 88.9% dan petiol 100%. Berat kalus tertinggi eksplan daun 0.157 gram dan petiol 0.103 gram. Eksplan daun pada IBA 3 mg/l menghasilkan kadar stigmasterol dan sitosterol tertinggi yaitu 1242.9 ppm dan 2079.42 ppm, sedangkan eksplan petiol stigmasterol 897,69 ppm dan sitosterol 1225,6 ppm
ENGLISH:
Purwoceng (Pimpinella alpine Molk.) Is a Indonesian medicinal plant that has efficacy as an aphrodisiac, diuretic and tonic. The existence of this plant has been consideredrare and endangered. Tissue culture techniques can be used as an alternative means of propagation and secondary metabolite production. In this study, plant growth regulators IBA (Indole Butyric Acid) is used in the media MS for callus induction and increasing levels of secondary metabolites such as stigmasterol and sitosterol. Intention of this research to know influence of type of explants and concentration IBA of growth and secondary metabolite levels (stigmasterol and sitosterol) callus Purwoceng (Pimpinella alpine Molk.) In the media MS.
This study included in the experimental with complete random device (RAL) factorial, treatment in the form of type explants and IBA concentration. Explants are in the form of leaf and petiol while IBA concentrations used were 0 mg/l, 3 mg/l, 5 mg/l and 7 mg/l. Test levels of stigmasterol and sitosterol were performed by using column chromatography. Observational data in the form of qualitative data (visual observation including day appeared callus, callus morphology and percentage of callus) and quantitative (callus weight, levels of sitosterol and stigmasterol). Qualitative data were analyzed by using descriptive analysis while quantitative data were analyzed by ANOVA (analysis of variance) 2 way, if there is influence hace continued with DMRT 5%.
The results of research showed that the are real influence of type explant and IBA concentrations of growth and secondary metabolite levels (stigmasterol and sitosterol) callus Purwoceng. Leaf explants with treatment IBA 3 mg/l was the best treatment which produces the fastest growth 10 hst has emerged callus chromatic yellowish green with a compact texture, and the highest percentage of callus growth on leaves 88.9% and petiols 100%. The highest callus weight produced is 0.157 grams for the leaves and petiol 0.103 grams. Leaf explants on IBA 3 mg/l produced the highest levels of stigmasterol and sitosterol. That is 1242.9 and 2079.42 ppm ppm, while petiol explants stigmasterol 897,69 ppm and sitosterol 1225,6 ppm.
Item Type: | Thesis (Undergraduate) |
---|---|
Keywords: | IBA (Indole Butyric Acid); Kalus; Purwoceng (Pimpinella alpine Molk.); Sitosterol; Stigmasterol; IBA (Indole Butyric Acid); Callus; Sitosterol; Stigmasterol |
Subjects: | 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology |
Departement: | Fakultas Sains dan Teknologi > Jurusan Biologi |
Depositing User: | Saputra Edi |
Date Deposited: | 28 Jul 2015 11:34 |
Last Modified: | 28 Jul 2015 11:34 |
URI: | http://etheses.uin-malang.ac.id/id/eprint/578 |
Downloads
Downloads per month over past year
Actions (login required)
![]() |
View Item |