Pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L) terhadap kadar Superoksida Dismutase (SOD) dan Malondialdehide (MDA) hepar mencit betina (Mus musculus) yang diinduksi 7,12-dimetilbenz (α) antrasen secara in vivo

Chori, Finka Aidila Mifatisul (2013) Pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L) terhadap kadar Superoksida Dismutase (SOD) dan Malondialdehide (MDA) hepar mencit betina (Mus musculus) yang diinduksi 7,12-dimetilbenz (α) antrasen secara in vivo. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.

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Abstract

INDONESIA:

Adanya zat radikal bebas yang berlebihan menyebabkan terjadinya stress oksidatif. Tingginya stress oksidatif, menyebabkan peningkatan aktivitas radikal bebas dalam tubuh dan menurunkan aktivitas antioksidan enzimatis. Aktivitas radikal bebas tersebut hanya dapat dihambat oleh adanya antioksidan. Salah satu tanaman yang telah terbukti mengandung antioksidan adalah daun sirsak (Annona muricata L.). Kandungan daun sirsak yang bersifat sebagai antioksidan adalah flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L.) terhadap kadar Superoksida Dismutase (SOD) dan Malondialdehide (MDA) hepar mencit betina yang diinduksi 7,12- dimetilbenz (α) antrasen secara in vivo.

Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan empat kali ulangan. Perlakuan yang digunakan adalah pemberian ekstrak etanol daun sirsak (Annona muricata L.) dengan empat dosis, yaitu dosis I (100 mg/kg BB/oral/hari), dosis II (150 mg/kg BB/oral/hari), dosis III (200 mg/kg BB/oral/hari) dan dosis IV (250 mg/kg BB/oral/hari). Untuk mengukur aktivitas antioksidan enzimatis, parameter yang digunakan adalah kadar Superoksida Dismutase (SOD). Untuk mengukur aktivitas radikal bebas, parameter yang digunakan adalah kadar Malondialdehide (MDA). Data yang didapat dianalisis menggunakan ANOVA dengan taraf signifikansi 1%.

Hasil penelitian menunjukkan bahwa pemberian ekstrak etanol daun sirsak (Annona muricata L) berpengaruh terhadap peningkatan kadar SOD dan penurunan kadar MDA pada hepar mencit betina (Mus musculus) yang diinduksi 7,12-dimetilbenz (α) antrasen (p < 0,05). Dari keempat dosis ekstrak etanol daun sirsak yang diberikan, dosis 3 (200 mg/kg BB/oral/hari) merupakan dosis yang paling efektif dalam meningkatkan kadar SOD(99,6 U/ml) hingga mendekati kadar normal (135,4 U/ml) dan mampu menurunkan kadar MDA (16,607 nmol/ml) hingga mendekati kadar normal (8,25 nmol/ml).

ENGLISH:

The presence of excessive free radicals that cause oxidative stress. The high oxidative stress, causing an increase in free radical activity in the body and lower the antioxidant enzymatic activity. Free radical activity can only be inhibited by the presence of antioxidants. One of the plants that have been shown to contain antioxidants is the leaves of Annona muricata L. The content that is Annona muricata leaves as antioxidants are flavonoids. This study aimed to determine the effect of ethanol extract of leaves of Annona muricata L. on levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) female mice liver induced by 7,12 - dimetilbenz (α) antrasen in vivo.

This research used Completely Randomized Design (CRD) with six treatments and four replications. The treatment used is ethanol extract of leaves of Annona muricata L. with four doses, the first dose (100 mg / kg / oral / day), II dose (150 mg / kg / oral / day), doses III (200 mg / kg / oral / day) and IV dose (250 mg / kg / oral / day). To measure the enzymatic antioxidant activity, the parameters used are the levels of superoxide dismutase (SOD). To measure the activity of free radicals, the parameters used are the levels of malondialdehyde (MDA). The data were analyzed using ANOVA with a significance level of 1%.

The results showed that the ethanol extract of leaves of Annona muricata L. effect on increased levels of SOD and decreased MDA levels in the liver of female mice (Mus musculus) induced by 7.12-dimetilbenz (α)antrasen (p < 0,05). Four doses of ethanol extract of leaves of Annona muricat is given, 3 doses (200 mg / kg / oral / day) is the most effective dose in increasing levels of SOD (99.6 U / ml) to near normal levels (135.4 U / ml) and is able to reduce levels of MDA (16.607 nmol / ml) to near normal levels (8.25 nmol / ml).

Item Type: Thesis (Undergraduate)
Supervisor: Susilowati, Retno and Abidin, Munirul
Keywords: Annona muricata; SOD; MDA; Hepar; 7,12-Dimetilbenz (α) Antrasen; Annona muricata; SOD; MDA; Liver; 7,12-Dimetilbenz (α) Antrasen
Subjects: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060806 Animal Physiological Ecology
Departement: Fakultas Sains dan Teknologi > Jurusan Biologi
Depositing User: Chusnul Faida Ulfa
Date Deposited: 28 Jul 2015 04:37
Last Modified: 28 Jul 2015 04:37
URI: http://etheses.uin-malang.ac.id/id/eprint/574

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