Pengaruh enzim kitinase kasar dari bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae terhadap pertumbuhan, morfologi, dan kadar N-asetilglukosamin Fusarium oxysporum

Ifnawati, Khoir (2013) Pengaruh enzim kitinase kasar dari bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae terhadap pertumbuhan, morfologi, dan kadar N-asetilglukosamin Fusarium oxysporum. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.

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Abstract

INDONESIA:

Layu Fusarium merupakan salah satu penyakit yang disebabkan oleh jamur patogen. Fusarium oxysporum. Jamur ini adalah salah satu jamur patogen yang sulit dikendalikan. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian enzim kitinase kasar yang diisolasi dari bakteri Pseudomonas pseudomallei, Klebsiella ozaenae, dan kombinasi dari keduanya terhadap pertumbuhan, morfologi, dan kadar N-asetilglukosamin jamur F.oxysporum.

Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), 4 perlakuan dan 6 ulangan. Penelitian dilaksakan pada bulan Maret sampai Juni 2013 di Laboratorium Mikrobiologi, Genetika, dan Optik Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang. Data yang diperoleh dianalisis menggunakan ANOVA Oneway dan apabila perlakuan yang diberikan memberikan pengaruh maka dilanjutkan dengan uji Duncan dengan taraf 5%. Perlakuan yang digunakan adalah enzim kitinase dari bakteri Pseudomonas pseudomallei, dari bakteri Klebsiella ozaenae, dari kombinasi bakteri antara Pseudomonas pseudomallei dan Klebsiella ozaenae, serta kontrol.

Hasil penelitian menunjukkan bahwa semua perlakuan pemberian enzim kitinase baik dari bakteri Pseudomonas pseudomallei, Klebsiella ozaeae, maupaun kombinasi keduanya, dapat memberikan pengaruh bagi pertumbuhan, morfologi, dan kadar N-asetilglukosamin jamur Fusarium oxysporum. Pertumbuhan jamur yang paling terhambat adalah yang diberi enzim kitinase dari bakteri Pseudomonas pseudomallei, yaitu sebesar 12 mm pada hari ke-6 pengamatan. Abnormalitas morfologi miselium jamur Fusarium oxysporum tidak terlihat adanya perbedaan diantara ketiga perlakuan pemberian enzim kitinase, yaitu menjadi mengecil, membengkok, lisis, dan hancur. Kadar N-asetilglukosamin tertinggi yaitu perlakuan yang diberi enzim kitinase kasar dari bakteri Klebsiella ozaenae.

ENGLISH:

Fusarium wilt is a disease caused by the fungal pathogen Fusarium oxysporum. This fungus is one that is difficult to control fungal pathogens. This study was conducted to determine the effect of crude chitinase enzyme isolated from Pseudomonas pseudomallei bacterium, Klebsiella ozaenae, and a combination of both on growth, morphology, and levels of N-acetylglucosamine F.oxysporum.

This study was an experimental study using a completely randomized design (CRD), 4 treatments and 6 replications. The research was done in March to June 2013 in the Laboratory of Microbiology, Genetics, and Optical Science and Technology Faculty of the Islamic University of Maulana Malik Ibrahim Malang. The data were analyzed using ANOVA Oneway and if treatment is given effect then followed by Duncan's test at 5% level. The treatment used is the enzyme chitinase from Pseudomonas pseudomallei bacteria, from bacteria Klebsiella ozaenae, of combination among Pseudomonas pseudomallei bacterium and Klebsiella ozaenae, and control.

The results showed that all treatments providing both chitinase enzyme of Pseudomonas pseudomallei bacterium, Klebsiella ozaeae, and a combination of both, to give effect to the growth, morphology, and levels of N-acetylglucosamine Fusarium oxysporum. Most inhibited fungal growth is a given enzyme chitinase from Pseudomonas pseudomallei, which is equal to 12 mm on the 6th day of observation. Morphological abnormalities of the mycelium Fusarium oxysporum not seen any difference among the three treatment provision enzyme chitinase, which may shorten, bend, lysis, and destroyed. Highest levels of N- acetylglucosamine which treatment was given rough chitinase enzyme from the bacterium Klebsiella ozaenae.

Item Type: Thesis (Undergraduate)
Supervisor: Utami, Ulfah and Abidin, Munirul
Keywords: Bakteri Pseudomonas pseudomallei; Klebsiella ozaenae; Fusarium oxysporum; Enzim Kitinase; N-asetilglukosamin; Anti Jamur; morfologi; Pseudomonas pseudomallei Bacteria; Chitinase Enzyme; N-acetylglucosamine; Anti-Fungal; Morphology
Subjects: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060505 Mycology
Departement: Fakultas Sains dan Teknologi > Jurusan Biologi
Depositing User: Saputra Edi
Date Deposited: 28 Jul 2015 02:35
Last Modified: 28 Jul 2015 02:35
URI: http://etheses.uin-malang.ac.id/id/eprint/531

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