Uji aktivitas antitumor ekstrak metanol benalu teh (Scurulla atropurpurea) pada kulit mencit (Mus musculus) yang diinduksi 7,12-dimethilbenz(α)antrasen (DMBA) secara invivo

'Ulumi, Afrilia Mamlu'atul (2013) Uji aktivitas antitumor ekstrak metanol benalu teh (Scurulla atropurpurea) pada kulit mencit (Mus musculus) yang diinduksi 7,12-dimethilbenz(α)antrasen (DMBA) secara invivo. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.

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Abstract

INDONESIA:

Benalu teh (Scurrula atropurpurea Bl. Dans) adalah tumbuhan parasit dari famili Loranthaceae yang tumbuh pada pohon teh sebagai tumbuhan inangnya. Scurrulla atropurpurea secara tradisional telah dikenal berfungsi sebagai alternatif obat berbagai macam penyakit salah satunya sebagai anti tumor, karena tumbuhan ini mangandung senyawa aktif golongan flavonoid yaitu kuersetin sebagai penekan tumor. Penelitian ini bertujuan untuk mengetahui aktivitas antitumor ekstrak metanol benalu teh (Scurulla atropurpurea) pada kulit mencit (Mus musculus) yang diinduksi 7,12- dimethilbenz(α)antrasen (DMBA) secara invivo.

Penelitian ini bersifat eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan 6 kali ulangan. Beberapa perlakuan tersebut diantaranya adalah mencit yang diinduksi DMBA 25μg/100μl aseton yang diikuti dengan pemberian ekstrak metanol benalu teh dosis 0mg/KgBB (K+), 750mg/KgBB (P1), 1500mg/KgBB (P2), dan 2250mg/KgBB (P3). Hewan coba yang digunakan adalah mencit jantan galur Balb/c, berumur 4-6 minggu dan berat 15-20 gram. Parameter yang diamati adalah preparat histologi jaringan kulit yang terkena tumor ditandai dengan adanya displasia dan ketebalan lapisan kulit, kejadian tumor yang meliputi insidensi tumor, volume nodul, luas permukaan luka dan kerontokan bulu, serta perubahan berat badan mencit. Data ketebalan lapisan kulit yang diperoleh dianalisis dengan ANAVA satu arah. Apabila terdapat perbedaan sangat nyata maka dilanjutkan dengan uji duncan (α 1%).

Aktivitas antitumor ekstrak metanol benalu teh (Scurulla atropurpurea) pada kulit mencit (Mus musculus) yang diinduksi DMBA dapat mempengaruhi ketebalan lapisan kulit, kejadian tumor meliputi insidensi tumor, mengurangi volume nodul, luas permukaan luka dan kerontokan bulu, serta perubahan berat badan mencit. Dosis ekstrak benalu teh yang efektif pada penurunan ketebalan lapisan kulit mencit adalah 750mg/KgBB.

ENGLISH:

Tea parasite (Scurrula atropurpurea BI. Dans) is plant from the family Loranthaceae that grow on the tea tree as their host plants. Scurrulla atropurpurea has traditionally been known to function as an alternative remedy various diseases one of them as anti tumor because this plants contain active compounds of flavonoid quercetin as a tumor suppressor. This study aims to determine the antitumor activity of the methanol extract on tea parasite (Scurulla atropurpurea) in the skin of mice (Mus musculus) induced 7.12-dimethilbenz (a) antrasen (DMBA) in vivo.

This is an experimental study using a completely randomized design (CRD) with 4 treatments 6 replications. Some of these treatments include DMBA-induced mice 25ug/100ul acetone followed by methanol extract of tea parasite dose Omg / KgBW (K+), 750mg/KgBB (Pl), 1500mg/KgBB (P2), and 2250mg/KgBB (P3) . Experimental animals used were male mice strain Balb / c, 4-6 weeks old and weighing 15-20 grams. Parameters measured were preparations tissue histology of tumors characterized by the presence of dysplasia and skin layer thickness, the incidence of tumors including tumor incidence, nodule volume, fur loss and wound broad, as well as changes in body weight of mice. Skin layer thickness data were analyzed by one-way ANOVA. If there is a very real difference then followed by Duncan test (a1%).

Antitumor activity of methanol extract of tea parasite (Scurulla atropurpurea) in the skin of mice (Mus musculus) the induced DMBA can affect skin layer thickness, the incidence of tumors include tumor incidence, reducing the volume of the nodules, fur loss and wound surface area, and body weight changes in mice. Dose of parasite extracts were effective at decreasing the thickness of the epidermal layer of the skin of mice was 750mg/KgBB.

Item Type: Thesis (Undergraduate)
Supervisor: Holil, Kholifah and Syarifah, Umaiyatus
Keywords: Benalu teh (Scurulla atropurpurea); Kejadian tumor kulit; DMBA; mencit; Tea parasite (Scurrula atropurpurea); incidence of skin; DMBA; Mice
Subjects: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060806 Animal Physiological Ecology
Departement: Fakultas Sains dan Teknologi > Jurusan Biologi
Depositing User: Chusnul Faida Ulfa
Date Deposited: 27 Jul 2015 01:25
Last Modified: 27 Jul 2015 01:25
URI: http://etheses.uin-malang.ac.id/id/eprint/478

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