Pengaruh infusa daun murbei (Morus alba L.) terhadap gambaran histologi hipokampus tikus putih (Rattus norvegicus) model diabetes melitus kronis yang diinduksi aloksan

INDONESIA:

Daun murbei (Morus alba L.) merupakan salah satu tanaman tradisional yang mengandung senyawa antihiperglikemik dan antioksidan. Senyawa ini diketahui mampu menurunkan kadar glukosa darah yang tinggi dan memperbaiki kerusakan organ akibat peningkatan radikal bebas karena kondisi hiperglikemik. Penelitian ini bertujuan untuk mengetahui pengaruh infusa daun murbei (Morus alba L.) terhadap gambaran histologi sel pyramid hipokampus tikus putih (Rattus norvegicus) model diabetes melitus kronis yang diinduksi aloksan.

Penelitian ini bersifat eksperimental yang menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan 4 kali ulangan. Perlakuan yang digunakan yaitu K (+) (kontrol positif), K (-) (kontrol negatif), P1 (400 mg/kgBB ), P2 (600 mg/kg BB), P3 (800 mg/kg BB) dan P4 (1000 mg/kg BB). Hewan yang digunakan adalah tikus putih strain Wistar sebanyak 24 ekor yang berumur 2 bulan dengan berat rata-rata 100-200 gram. Data hasil penelitian meliputi jumlah sel pyramid CA1 Hipokampus. Data yang diperoleh dianalisis dengan ANOVA One Way signifikansi 0,01 (1%), apabila terdapat perbedaan sangat nyata maka dilanjutkan dengan uji duncan α 5%.

Hasil penelitian menunjukkan bahwa pemberian infusa daun murbei (Morus alba L.) berpengaruh terhadap gambaran histologis sel pyramid hipokampus tikus putih (Rattus norvegicus) dan dosis optimal infusa daun murbei yang berpengaruh terhadap jumlah sel pyramid CA1 hipokampus tikus putih (Rattus norvegicus) yang diinduksi aloksan adalah 1000 mg/kg BB.

ENGLISH:

Leaves of mulberry (Morus alba L.) is a traditional plant which has antihyperglycemic and antioxidant. Those compounds function are to decrease the high blood glucose level and to repair the damage of organ from free radical which is caused by hyperglycemic condition. The aim of this research is to know the effect of Mulberry leaves (Morus alba L.) infusion to the histology picture of hippocampus white mouse (Rattus norvegicus) which is inducted by Alloxan.

This is an experimental research that uses complete random program with 6 different treatment and 4 times repetition. The different treatment are K (+) positive control, K (-) negative control, P1 (400 mg/kg weight), P2 (600 mg/kg weight), P3 (800 mg/kg weight), and P4 (1000 mg/kg weight). Animal that is used in this research is 24 heads of Strain Wistar white mouse, 2 month with average- weight 100-200 grams. The research data are pyramid cell amount. In addition, the data is analyzed by ANOVA One Way with 0,01 (1%) significance, if there are very real different the research is continued by advance duncan α 1% test.

The result of research shows that Mulberry leaves (Morus alba L.) infusion are very real influence to re-increase pyramid cell amount to the mouse which inducted by alloxan. The optimal doses of Mulberry leaves (Morus alba L.) infusion that influence for increasing pyramid cell CA1 amount of inducted by alloxan mouse is P4 (1000 mg/kg weight).