Widayanti, Farrah Nur (2019) Pengaruh ekstrak kombinasi Cinnamomum burmannii dan Eleutherine bulbosa terhadap kadar kolesterol total secara in vivo dan in silico. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
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Abstract
INDONESIA:
Dislipidemia merupakan suatu keadaan dimana terjadi ketidaknormalan profil lipid dalam tubuh. Dislipidemia merupakan faktor resiko utama dari penyakit kardiovaskuler. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian ekstrak etanol 96% Cinnamomum burmannii dan Eleutherine bulbosa terhadap kadar kolesterol total serum darah mencit secara in vivo dan in silico. Uji in vivo merupakan penelitian eksperimental laboratorium menggunakan RAL sebanyak 8 perlakuan dan 4 ulangan. Kelompok perlakuan antara lain adalah N (Normal), K- (HFD), K+ (HFD + atorvastatin), P1 (HFD + 100% C. burmannii), P2 (HFD + 75% C. burmannii& 25% E. bulbosa), P3 (HFD + 50% C. burmannii& 50% E. bulbosa), P4 (HFD + 25% C. burmannii& 75% E. bulbosa), dan P5 (HFD + 100% E. bulbosa). Induksi HFD diberikan selama 120 hari dan pemberian terapi dilakukan pada hari ke-91 hingga 120. Analisis data menggunakan ANOVA dengan α=5%. Hasil analisis ANOVA menunjukkan bahwa pemberian ekstrak C. burmannii dan E. bulbosa serta kombinasi keduanya dapat menurunkan kadar kolesterol total serum darah mencit yang diinduksi HFD. Uji in silico pada penelitian ini menggunakan metode molecular docking ligan senyawa aktif yang terkandung pada C. burmannii dan E. bulbosa dengan reseptor HMG KoA reduktase. Hasil molecular docking yang dikaitkan dengan prediksi PASS, HIA, dan kalkulasi nilai drug-likeness menunjukkan bahwa senyawa quercetin dan eleutherine merupakan senyawa yang paling berpotensi dalam penurunan kadar kolesterol total melalui penghambatan HMG KoA reduktase.
ENGLISH:
Dyslipidemia is a condition of lipid profile abnormality in the body. Dyslipidemia is a major risk factor for cardiovascular disease. The purpose of this study is to determine the effect of 96% ethanolic extract administration of Cinnamomum burmannii and Eleutherine bulbosa on total cholesterol levels of mice serum blood by in vivo and in silico. In vivo test was an experimental laboratory study using CRD for eight treatments and four replications. The treatment group included N (Normal), K- (HFD), K+ (HFD + atorvastatin), P1 (HFD + 100% C. burmannii), P2 (HFD + 75% C. burmannii& 25% E. bulbosa), P3 (HFD + 50% C. burmannii& 50% E. bulbosa), P4 (HFD + 25% C. burmannii& 75% E. bulbosa), and P5 (HFD + 100% E. bulbosa). HFD induction was given for 120 days, and therapy was given on days 91 to 120. Data analysis was using ANOVA with α=1%. ANOVA analysis results showed that the administration of C. burmannii and E. bulbosa extracts as well as a combination of both could reduce total serum blood cholesterol levels of HFD-induced mice. In silico test in this study used the molecular docking ligand methods of active compounds contained in C. burmannii and E. bulbosa with HMG-CoA reductase receptors. The results of molecular docking associated with the prediction of PASS, HIA, and calculation of drug-likeness values indicated that the quercetin and eleutherinol compound was the most potential compound in reducing total cholesterol levels through inhibition of HMG-CoA reductase.
Item Type: | Thesis (Undergraduate) | |||||||||
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Supervisor: | Susilowati, Retno and Prasetyo, Oky Bagas | |||||||||
Contributors: |
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Keywords: | Kolesterol total, Serum darah, Induksi HFD, Ekstrak, Total cholesterol, Blood serum, HFD induction, Extract, in vivo, in silico, Molecular docking | |||||||||
Departement: | Fakultas Sains dan Teknologi > Jurusan Biologi | |||||||||
Depositing User: | Zulaikha Zulaikha | |||||||||
Date Deposited: | 12 Jun 2020 14:08 | |||||||||
Last Modified: | 12 Jun 2020 14:08 | |||||||||
URI: | http://etheses.uin-malang.ac.id/id/eprint/17797 |
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