Pramitania, Vioreta Aprilia (2019) Uji toksisitas isolat steroid hasil kromatografi kolom fraksi n-Heksana Alga Merah (Eucheuma cottonii) dari Perairan Wongsorejo Banyuwangi. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
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Abstract
INDONESIA :
Alga merah Eucheuma cottonii memiliki kandungan senyawa metabolit sekunder dengan manfaat yang beragam. Senyawa steroid merupakan salah satu senyawa metabolit sekunder dengan tingkat toksisitas yang tinggi. Penelitian ini dilakukan dengan tujuan untuk mengetahui tingkat toksisitas isolat steroid fraksi n-heksana dan mengetahui jenis steroid pada alga merah Eucheuma cottonii.
Isolasi Eucheuma cottonii dilakukan dengan ekstraksi maserasi pelarut metanol. Ekstrak kasar yang didapat dihidrolisis dengan HCl 2 N dan dipartisi dengan pelarut n-heksana. Fraksi n-heksana diuji fitokimia dengan reagen Liebermann-Burchard. Kemudian diisolasi menggunakan kromatografi kolom dengan gradien eluen n-heksana:etil asetat (95:5, 90:10, 85:15, 80:20, 75:25, 70:30). Senyawa steroid yang terpisahkan dengan baik dilakukan uji toksisitas metode BSLT pada konsentrasi 1 ppm, 2 ppm, 3 ppm, 4 ppm, dan 5 ppm. Isolat dengan nilai toksik tertinggi diidentifikasi menggunakan UV-Vis, FTIR dan LCMS/MS.
Fraksi n-heksana yang diuji fitokimia dengan reagen Liebermann-Burchard menghasilkan cincin hijau yang menandakan positif mengandung steroid. Hasil pemisahan kromatografi kolom senyawa steroid memberi nilai LC50 sebesar 11,0746 ppm pada uji toksisitas BSLT. Identifikasi menggunakan UV-Vis memberi serapan 203 nm dan 276 nm. Hasil identifikasi FTIR menunjukkan serapan khas steroid OH dan C-O alkohol sekunder. Hasil LCMS/MS isolat menunjukkan adanya steroid jenis sitosterol, fukosterol, kolesterol dan demosterol.
ENGLISH :
Red algae Eucheuma cottonii contains secondary metabolites with various benefits. Steroid compounds are one of the active secondary metabolites with high toxicity. This study was conducted with the aim to determine the toxicity level of n-hexane fraction steroid isolates and determine the type of steroids in red algae Eucheuma cottonii.
Macroalgae Eucheuma cottonii has steroid content with a good level of toxicity. Isolation of Eucheuma cottonii was carried out with methanol solvent maceration extraction. The crude extract obtained was hydrolyzed with HCl 2 N and partitioned with n-hexane. Furthermore, it was isolated using column chromatography with n-hexane: ethyl acetate eluent gradient (95:5, 90:10, 85:15, 80:20, 75:25, 70:30). The results monitored by ATLC. The best result of separation by ATLC was tested for toxicity with the BSLT method at a concentration of 1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm. Isolates with the highest toxic levels were identified by UV-Vis and FTIR
The n-hexane fraction tested by phytochemical with the Liebermann-Burchard reagent, and produced a green ring which indicated positive steroid content. The results of column chromatography separation in steroid compounds which gave LC50 values of 11.0746 ppm in the BSLT toxicity test. Identification using UV-Vis gives absorption of 203 nm and 276 nm. The results of FTIR identification showed a typical absorption of steroid OH, and secondary alcohols in C-O. Isolate LCMS/MS results showed the presence of sitosterol steroids, fukosterol, cholesterol and demosterol.
Item Type: | Thesis (Undergraduate) | |||||||||
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Supervisor: | Fasya, A. Ghanaim and Imamudin, Mochamad | |||||||||
Contributors: |
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Keywords: | Eucheuma cottonii; kromatografi kolom; steroid; uji toksisitas; column chromatography; steroid; toxicity test | |||||||||
Departement: | Fakultas Sains dan Teknologi > Jurusan Kimia | |||||||||
Depositing User: | Dian Anesti | |||||||||
Date Deposited: | 05 Mar 2020 10:11 | |||||||||
Last Modified: | 09 Jun 2023 15:01 | |||||||||
URI: | http://etheses.uin-malang.ac.id/id/eprint/15259 |
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